CLL immunophenotypic score (Matutes score) Immunophenotypic score for diagnosis of chronic lymphocytic leukemia Flow cytometric analysis of peripheral blood or bone marrow is performed for expression of the cell surface markers listed in the table below. The scores for each marker are summed. Value Points Cell surface marker 0 points 1 point CD79b (or CD22) -Please Select Strong Weak CD79b (or CD22) Strong Weak CD23 -Please Select Negative Positive CD23 Negative Positive CD5 -Please Select Negative Positive CD5 Negative Positive FMC7 -Please Select Negative Positive FMC7 Positive Negative SmIg -Please Select Strong Weak SmIg Strong Weak Sum of score A score ≥ 4 is indicative of CLL. A score of ≤ 3 should prompt consideration of an alternative diagnosis, Diagnosis References 1. Matutes E, Owusu-Ankomah K, Morilla R, Garcia Marco J, Houlihan A, Que TH et al. The immunological profile of B-cell disorders and proposal of a scoring system for the diagnosis of CLL. Leukemia 1994; 8: 1640-5. 2. Moreau EJ, Matutes E, A’Hern RP, Morilla AM, Morilla RM, Owusu-Ankomah KA et al. Improvement of the chronic lymphocytic leukemia scoring system with the monoclonal antibody SN8 (CD79b). Am J Clin Pathol 1997; 108: 378-382.